| Resumen |
- La patogenicidad de nematodos entomopatógenos fue evaluada contra Anastrepha luden (Loew); en condiciones de laboratorio. Al aplicar 2000 Jls/ml a larvas del tercer estadio de A. Iudens se encontró que Heterorhabditis indica presentó una mortalidad acumulada en larvas y pupas de A. tudens del 71.13 porciento, S. carpocapsae cepa Mexicana 53.03 porciento, Steinernema carpocapsae cepa All 51.2 porciento, H. bacteriophora HP88 47.29 porciento y Steinernema riobrave con 38.26 porciento. Las especies H. indica, S. carpocapsae cepas Mexicana y AII y fueron evaluadas a las concentraciones de 0, 1000,2000,3000,4000,5000,6000 Jls/ml, presentando H. indica un CL50 de 7.23; S. carpocapsae cepa Mexicana 19.31 y S. carpocapsae cepa All 33.22 nematodos cm 2 /suelo. Este estudio demostró variabilidad en la patogenicidad de las diferentes especies y razas de nematodos evaluados en contra de estados inmaduros de A. Iudens; donde H. indica, S. carpocapsae cepas Mexicana y AII muestran tener potencial para ser usadas como agentes de control biológico de la plaga. The pathogenicity of entomopathogenic nematode was evaluated against Anastrepha luden (Loew); under laboratory conditions. Cumulative mortality of larvae and pupae was about 71.13 percent with the concentration of 2000 lJs/ml using the Heterorhabditis indica . Meanwhile, S. carpocapsae Mexican Steinernema carpocapsae AH, H. bacteriophora HP88 and Steinernema riobrave caused 53.03,51.2, 42.29, and 38.26 percent of cumulative mortality, respectively. H. indica, S. carpocapsae Mexican AII and strains were evaluated using the following concentrations: 0,1000,2000,3000,4000,5000, 6000 Jls/ml. Mean lethai concentrations (LC50) for H. indica was 7.23, and S. carpocapsae Mexican and Al strains viere 19.31 and 33.22 nematodes /cm 2 Variability in the expression of pathogenicity was demonstrated between species and strains of the entomopathogenic nematode A. luden larvae and pupae. H. índica, S. carpocapsae Mexican and AII strains exhibited potential as biological control against the thirth instar larvae and pupae of Mexican fruit fly. |
Tesis de posgrado